Inverter

Part:BBa_K223058

Designed by: Anusuya Ramasubramanian   Group: iGEM09_Stanford   (2009-10-18)


RBS+mutTrpR+TT+mutTrp Operator

This part is a combination of K223051 and K223052. It is an inverter that is sensitive to concentration decreases in 5-methyl-L-tryptophan and converts this to a PoPs output signal used for downstream transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 534
    Illegal suffix found in sequence at 368
    Illegal SpeI site found at 683
    Illegal PstI site found at 697
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 534
    Illegal SpeI site found at 369
    Illegal SpeI site found at 683
    Illegal PstI site found at 383
    Illegal PstI site found at 697
    Illegal NotI site found at 27
    Illegal NotI site found at 376
    Illegal NotI site found at 540
    Illegal NotI site found at 690
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 21
    Illegal EcoRI site found at 534
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 21
    Illegal suffix found in sequence at 369
    Illegal EcoRI site found at 534
    Illegal XbaI site found at 549
    Illegal SpeI site found at 683
    Illegal PstI site found at 697
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 21
    Illegal EcoRI site found at 534
    Illegal XbaI site found at 36
    Illegal XbaI site found at 549
    Illegal SpeI site found at 369
    Illegal SpeI site found at 683
    Illegal PstI site found at 383
    Illegal PstI site found at 697
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 215


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